Fourier transform infrared (FTIR) microscopy is a promising analytical tool for detecting changes in cells due to viral infection.
In this study we have used FTIR microscopy to study cells that have been infected with four different viruses that infect humans and animals. The four viruses are herpes simplex type 1 (HSV1), adenovirus type 5 (Ad-5), coxsackie virus and measles virus.
In this report, we focus on cells that have been infected for 24 hours with either HSV1 or Ad-5. Our results show significant differences in spectra obtained using normal uninfected cell samples (mock), compared to cell samples infected with HSV1 or Ad-5.
To estimate how well spectra for uninfected and infected samples could be discriminated we have used advanced statistical methods (Hierarchical cluster analysis (HCA), Graphic analysis, logistic and partial least squares regression).

Keywords: virus; FTIR microscopy